Cadmium partitioning between hulls and kernels in three sunflower varieties: consequences for food/feed chain safety.

Contamination of sunflower seeds with soil Cd is an important issue for food and feed because this species strongly accumulates this metal. The present work reports that seeds from three sunflower varieties (ES Biba, Extrasol, Vellox) cultivated in the field in a calcareous agricultural soil having a moderately high Cd content (1 mg Cd/kg) had Cd contents of 0.84, 0.88 and 0.76 mg Cd/kg, respectively, all exceeding the regulation limit of 0.5 mg Cd/kg seeds for human food. On average, for the three varieties, washing seeds did not affect their total Cd contents but slightly increased the Cd in the kernels at the expense of that in hulls. Despite the Cd content of the whole seeds not differing between the varieties, the Cd fraction in the edible kernel differed significantly between varieties from 78 to 87% of the total seed Cd. The results of this study suggest that (i) the size of the kernel, relative to that of the hull, may affect the dilution of Cd in kernel tissues and (ii) there might be genetic variability for the capacity of transfer of Cd from the hull to the kernel. This opens the perspective to increase food safety by selecting sunflower genotypes that retain more Cd into the hull and transfer less of it to the edible kernel.

Digestive and metabolic bioavailability in healthy humans of 15N-labeled rapeseed and flaxseed protein incorporated in biscuits.

BACKGROUND: In the search to diversify protein sources for humans, oilseeds are good candidates due to the high protein content of their coproducts after oil extraction. Among them, rapeseed presents a well-balanced amino acid (AA) profile. Flaxseed is an emerging source but the nutritional value of its protein is not yet documented. OBJECTIVES: This study aimed to determine the nitrogen (N) and AA bioavailability of these protein sources. METHODS: Nineteen healthy volunteers were intubated with a naso-ileal tube. They ingested 156 g biscuits containing intrinsically labeled 15N rapeseed (n = 10) or flaxseed (n = 9) protein over a 4-h period. Ileal digesta, blood, and urine were sampled over 8 h after the first meal ingestion. N and 15N enrichment and AAs were measured to determine digestive and deamination losses. Ileal digestibility, the digestible indispensable AA score (DIAAS) and net postprandial protein utilization (NPPU) were calculated. RESULTS: Real ileal digestibility was 80.7 ± 6.5% for rapeseed protein and 92.2 ± 2.0% for flaxseed protein (P = 0.0002). Mean indispensable AA (IAA) digestibility reached 84.1 ± 6.9% and 93.3 ± 6.7% for rapeseed and flaxseed, respectively, lysine being the lowest digestible IAA for both sources. Despite moderate digestibility, the DIAAS was 1.1 for rapeseed but only 0.6 for flaxseed due to lysine insufficiency. Deamination losses accounted for 20.0 ± 6.5% of dietary N for flaxseed and 11.0 ± 2.8% for rapeseed (P = 0.002). The NPPU did not differ between the protein sources, with 71.3 ± 6.5% for flaxseed and 69.7 ± 7.6% for rapeseed. CONCLUSIONS: Despite good digestibility, flaxseed protein cooked in biscuits was penalized by both lysine insufficiency and poor lysine digestibility that decreased its DIAAS and increased deamination. By contrast, rapeseed was moderately digestible but presented no limiting IAA, resulting in an excellent DIAAS and low deamination. This study was registered at clinicaltrials.gov as NCT04024605.

Life cycle assessment data of French organic agricultural products.

Environmental data on organic products are needed to assess their environmental performance. The purpose of the ACV Bio project reported here was to generate environmental data as life cycle assessment (LCA) data for a sample of French organic production systems including cropping systems (annual crops, intercrops, forages), grassland, wine grapes, cow milk, calves, beef cattle, sheep, pigs, broilers and eggs. LCA was used to estimate environmental impacts of products from these systems. Recommended uses are to characterize part of the diversity of French organic farming systems and some of their environmental impacts, identify areas for improvement, perform eco-design and sensitivity analysis, and/or make system choices in a given context. However, these data do not represent average French organic products and should not be used as such. The MEANS-InOut web application was used to generate life cycle inventories (LCI). Impact assessment was performed using SimaPro v9 software. The Environmental Footprint 2.0 characterisation method was used to generate LCA data. These data were supplemented with three LCA indicators: cumulative energy demand, land competition (CML-IA non-baseline) and biodiversity loss. Three non-LCA indicators were also calculated for certain systems: diversity of crop families (for cropping systems), agro-ecological infrastructure (for sheep) and pesticide treatment frequency index (for grapes). In total, 173 products were modelled. LCA and non-LCA data are available in the Microsoft® Excel file at Data INRAE (https://doi.org/10.15454/TTR25S). LCI data are available in the AGRIBALYSE database and can be accessed using SimaPro and openLCA software. Farmer-practice data are available on demand.

Synthesis and Evaluation of Antioxidant Activities of Novel Hydroxyalkyl Esters and Bis-Aryl Esters Based on Sinapic and Caffeic Acids.

Novel hydroxyalkyl esters and bis-aryl esters were synthesized from sinapic and caffeic acids and aliphatic α,ω-diols of increasing chain lengths from 2 to 12 carbon atoms. Then, their antiradical reactivity (DPPH assay) and their antioxidant activity in a model oil-in-water emulsion (CAT assay) were evaluated. All the esters showed lower antiradical activities compared to their corresponding phenolic acid. This decrease was associated with the steric hindrance in hydroxyalkyl esters, and intramolecular interactions in bis-aryl esters. Regarding the two bis-aryl esters series in emulsion, the antioxidant capacity was improved with alkyl chain lengthening up to four carbons, after which it decreased for longer chains. This "cutoff" effect was not observed for both hydroxyalkyl esters series for which the alkyl chain lengthening results in a decrease of the antioxidant activity.

Experimental determination of pesticide processing factors during extraction of seed oils.

Processing Factors (PFs) reflect the concentration or dilution of pesticide residues resulting from food processing. PFs are key elements to demonstrate the compliance of processed foods with Maximum residue levels (MRLs) as set by Regulation 396/2005. While efforts have been made by the European Food Safety Authority (EFSA) and by national authorities to compile PFs from processing studies, such PFs are not available for all pesticides/processed product combinations. The EU vegetable oil and proteinmeal industry association (FEDIOL) has therefore developed a theoretical approach to approximate MRLs in crude vegetable oils and fats, based on the partition coefficient (log Pow) of the pesticides and on the oil content of the raw materials. To substantiate this approach, a pilot-scale processing study was initiated with rapeseeds spiked with selected pesticides and the experimental PFs for these pesticides determined. The aims of this study were (i) to study the reliability of pilot-scale conditions for PF determination and (ii) to assess the experimental PFs obtained in comparison to the theoretical PFs proposed by FEDIOL. This study demonstrated that production yields obtained for crude oil and meal in this processing study are similar to those in industrial processes even if differences were observed in the individual production steps (mechanical or solvent extraction steps). The experimental PFs obtained confirmed that the chosen fat-soluble pesticides did concentrate in the oil fraction. For metalaxyl-M having a log Pow lower than 3, a partitioning between the oil and the meal was observed, as expected. By comparing the experimental PFs and theoretical PFs, it can be concluded that the FEDIOL approach can be recommended as a suitable tool when PFs derived from specific processing studies are missing. Similar studies on pesticides with wider ranges of log Pow are required in order to complete our conclusions on default PFs for vegetable oils.

ECOALIM: A Dataset of Environmental Impacts of Feed Ingredients Used in French Animal Production.

Feeds contribute highly to environmental impacts of livestock products. Therefore, formulating low-impact feeds requires data on environmental impacts of feed ingredients with consistent perimeters and methodology for life cycle assessment (LCA). We created the ECOALIM dataset of life cycle inventories (LCIs) and associated impacts of feed ingredients used in animal production in France. It provides several perimeters for LCIs (field gate, storage agency gate, plant gate and harbour gate) with homogeneously collected data from French R&D institutes covering the 2005-2012 period. The dataset of environmental impacts is available as a Microsoft® Excel spreadsheet on the ECOALIM website and provides climate change, acidification, eutrophication, non-renewable and total cumulative energy demand, phosphorus demand, and land occupation. LCIs in the ECOALIM dataset are available in the AGRIBALYSE® database in SimaPro® software. The typology performed on the dataset classified the 149 average feed ingredients into categories of low impact (co-products of plant origin and minerals), high impact (feed-use amino acids, fats and vitamins) and intermediate impact (cereals, oilseeds, oil meals and protein crops). Therefore, the ECOALIM dataset can be used by feed manufacturers and LCA practitioners to investigate formulation of low-impact feeds. It also provides data for environmental evaluation of feeds and animal production systems. Included in AGRIBALYSE® database and SimaPro®, the ECOALIM dataset will benefit from their procedures for maintenance and regular updating. Future use can also include environmental labelling of commercial products from livestock production.